Transcend 16GB Class 10 SDHC Flash Memory Card compatible with Nikon d3100?

Will this memory card work in my Nikon D3100?

Sure, although it is a rather large capacity card.

I use a lot of Transcend memory cards, but none are larger than 8 gb, most are 4 gb.

Yes, but it's much more memory than you'll ever need. My everyday camera only has a 2GB card. I also have one 4GB & one 8GB card.
A 16GB card is more suited for videos.

Yes it will.